human normal liver cell lines thle 2  (Procell Inc)

Journal: Cancer Science

Article Title: Tumor exosomal RNPEP promotes lung metastasis of liver cancer via inducing cancer‐associated fibroblast activation

doi: 10.1111/cas.16417

Figure Lengend Snippet: RNPEP was overexpressed in hepatocellular carcinoma (HCC) tumors and cell lines. (A) RNPEP expression levels in human cancers in accordance with the data from Timer 2.0. (B) RNPEP expression levels in normal, HCC tumor and HCC metastatic tissues according to the data from the TNM plot. (C) RNPEP mRNA levels in normal human liver epithelial cells (THLE‐2), low‐metastatic HCC cells (MHCC‐97L), and high‐metastatic HCC cells (HCC‐LM3 and MHCC‐97H). Data were acquired by the real‐time PCR analysis and presented as mean ± SD, n = 3/group, * p < 0.05, ** p < 0.01. (D) RNPEP concentrations in cell supernatant of THLE‐2, MHCC‐97L, HCC‐LM3 and MHCC‐97H. Data were acquired by ELISA, and presented as the mean ± SD, n = 3/group, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: A normal human liver epithelial cell line (THLE‐2) was purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in cell‐specific culture medium (CM‐0833, Procell Life Science & Technology) at 37°C in a 5% CO 2 in air incubator.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay